Figure 1

Bursicon is expressed in type III boutons at the larval NMJ. Bursicon immunoreactivity (BURS-IR) has previously been shown in the periphery [10], without confirming its distribution pattern. (Aa) BURS-IR at a representative muscle 12 at the larval NMJ. (Ab) HRP immunoreactivity labels all bouton types at NMJ 12. (Ac) Bursicon distribution is found in some, but not all boutons at NMJ 12. (B) To verify if bursicon is expressed in type III boutons, we used transgenic larvae expressing the fluorescent neuropeptide marker ANF-EMD with a CCAP-GAL4 promoter. (Ba) BURS-IR at a representative muscle 12. (Bb) Vesicles with ANF-EMD are distributed in type III boutons. (Bc) BURS co-localizes with the ectopic ANF-EMD marker. Scale bars = 10 μm. An antibody which recognizes the bursicon-α subunit was consistently used for BURS-IR.