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Figure 3 | BMC Developmental Biology

Figure 3

From: Commitment of chondrogenic precursors of the avian scapula takes place after epithelial-mesenchymal transition of the dermomyotome

Figure 3

Epaxial epithelial dermomyotome grafted to the hypaxial domain gives rise to chondrocytes. A) Schematic illustration of the grafting procedure. Quail epaxial dermomyotome (blue) of somite 20/21 was transplanted to the hypaxial domain of chick embryo (red). dm, dermomyotome; n, neural tube; sc, sclerotome. B and C) Skeletal staining after six days of re-incubation. B) Control side shows normal scapula (s). C) Operated side shows a slim skeletal element at the level of the second thoracic segment (between arrows) connected to the remaining chick scapula (s). Vertebral column, rib and humerus (h) are normal. D and E) Immunohistochemistry on transverse sections through the operated region. D) Control side shows normal scapula (s), vertebral body (vb) and neural tube (n). E) Operated side exhibits a scapula of quail origin (black asterisk) and a normal rib (r) of chick origin. Insert in E) shows higher magnification of scapula with QCPN-positive cells.

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