Figure 4
Oct4-binding to Xist intron 1 decreases faster when differentiated in the presence of RA. Quantitative ChIP analyses of Oct4-binding to Xist intron 1 show faster reductions in Oct4-binding kinetics when wild-type and Tsix-deficient male cells were differentiated by EB+RA versus EB methods. Note that, for each experiment, we started with a single d0 (undifferentiated) culture for both X/Y and XΔ/Y, and then split them on subsequent days for analysis. Thus, d0 samples were the same for all EB series and for all EB+RA series, and their values were duplicated in the graphs for easier comparisons with d2, d4, d6 samples in the same series. Means ± s.e.m from two independent biological replicates are shown. Pairwise comparisons were made between corresponding samples (EB vs. EB+RA) normalized to the control IgG ChIP (background) for each time point. Statistical significance of each result was calculated using paired, two-tailed Student's t-tests. * p < 0.05.