Table 2 Oligonucleotide primers used for cDNA cloning or RT--PCR analysis
Gene (accession number) | Primer | Sequence | Position* |
|---|---|---|---|
ovSOLD1 and caSOLD1 | Forward | 5'--TCCAGAGATGGCTAAGTGCCTT--3' | 50--71 |
(NM_001105478) | Reverse | 5'--GAGTTGGACATGACTGAGCCAC--3' | 453--432 |
ovGAPDH | Forward | 5'--AAGGCCATCACCATCTTCCA--3' | 78--97 |
(AF030943) | Reverse | 5'--AGGTCAGATCCACAACGGACA--3' | 603--583 |
caGAPDH | Forward | 5'--GACCCCTTCATTGACCTTCAC--3' | 1--21 |
(AJ431207) | Reverse | 5'--TCATAAGTCCCTCCACGATGC--3' | 424--404 |