Chuzhoi mutants exhibit multiple developmental abnormalities. (A-D) E17.5 wild-type (A) and chuzhoi embryos (B-D) in lateral (A-C) and dorsal view (D). Chuzhoi embryos demonstrate the severe neural tube defect of craniorachischisis, where the neural tube is open from the midbrain/hindbrain boundary throughout the hindbrain and spinal cord, and the neural tissue is splayed on either side of the dorsal midline (D). Some chuzhoi mutants demonstrate failure to form closed eyelids (C) and a defect in ventral body wall development (likely omphalocele) with protrusion of the liver and guts (C). (E-L) Skeletal preparations of E17.5 wild-type (E, G, I) and chuzhoi mutant (F, H, J-L) fetuses stained with alcian blue for cartilage and alizarin red for bone. Dorsal views (E, F) demonstrate shortened and skewed body axis in chuzhoi mutants, with splayed vertebrae. Lateral views (G, H) reveal abnormal rib morphology, with fusions and bifurcations. Chuzhoi limbs sometimes exhibit polydactyly with an extra digit postaxially (J, arrow) or preaxially (K, L arrows).