Figure 4
From: Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment
The production of erythroid cells from treated hEBs. (A)Amplification of erythroid cells in liquid culture.50000 hEB cells from co-culture could yield about 5000,000 erythroid cells in liquid medium.(B) Analysis of erythroid differentiation of treated hEBs by flow cytometry. Erythroid cells derived from treated hEBs were seeded in liquid medium and tested for expression of CD71 (the marker of early erythroid), GLA (the marker of matuered erythroid) cells by flow cytometry at different inducing times. (C)Statistical analysis for the cell surface antigen expression of hEBs-derived erythroid cells in liquid medium. The data represent the mean ± SEM from three experiments. *, P < 0.05 when compared with the average expression of CD71 or GLA at day 4.