Figure 5

Characteristic morphology of various cell types in chicken retina at E18 with GFP labeling by in ovo electroporation technique. Expression of GFP is observed in all six cell types found in retina tissue through E18. Visualizing GFP expression at this stage shows cells localized in distinct layers and each of the cell type specific morphologies. Photoreceptors (A) have a cylindrical shape and are located in the outer nuclear layer (ONL). Horizontal cells (B) have processes located at the boundary of the inner nuclear layer (INL) and ONL with their cell bodies in the INL. Ganglion cells (C) are located in the GCL and have processes which mostly point toward the INL. Müller glial cells (D) span the entire retina with their cell bodies in the INL. Bipolar cells (E) have two distinct processes one that extends from the cell body in the INL to the ganglion cell layer (GCL) and the other to the ONL. Amacrine cells (F) have cell bodies in the INL and have processes that extent toward the GCL. OPL, outer plexiform layer; IPL, inner plexiform layer; Scale bar = 20 μm.