Skip to main content
Figure 4 | BMC Developmental Biology

Figure 4

From: Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques

Figure 4

Tracking development and migration of chicken embryonic retina cells using GFP labeling by in ovo electroporation technique. Chicken embryos are injected with pCAG-GFP and electroporated at embryonic day 4 (E4). GFP expression is observed during early stages of development, E7-E8 (A-B). These cells are elongated which is characteristics of cell migration. The cells span the whole width of the neural epithelial layer. In subsequent stages E9-E10 (C-D), cell layers begin to show distinct boundaries and cells begin to settle into their final layers. Cells also take on a rounder morphology and begin to extend their processes. The appearance of well defined cell type specific morphologies begins around E12 (E). Processes are more clearly visible and help to form clearly visible boundaries between layers. The clearest and most distinct and definitive cell morphologies are observed in GFP-expressing cells at E18 (F) (see Fig. 4). ONBL, outer neuroblastic layer; INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 50 μm.

Back to article page