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Figure 1 | BMC Developmental Biology

Figure 1

From: Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques

Figure 1

The expression of chick retinal cell-type specific marker determined by immunohistochemistry method. Retina tissue from chicken embryos were harvested at various time points during development, sectioned, and stained with retinal cell type specific antibodies Xap-1 (A-F), Visinin (G-L), Lim1+2 (M-R), and Brn3a (S-X). Xap-1 is known to selectively stain only the outer segments of photoreceptor cells, while Visinin is known to selectively stain the entire photoreceptor cells. Lim1+2 labels horizontal cells exclusively. Brn3a selectively labels as subset of ganglion cells. By staining retinas at various times during development, the onset of each cell type specific marker and their changes through out development were observed. ONBL, outer neuroblastic layer; INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 40 μm.

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