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Table 4 Time to aggregation at different cell densities.

From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development

Cells/plate

Cell density (x105 cells/cm2)

Wild-type

keaA -

2.5 × 107

4.4

9h

12 h

1.0 × 107

1.8

11 h

16 h

5.0 × 106

0.9

14 h

-

1.0 × 106

0.18

-

 
  1. Exponentially growing cells were washed, ressuspended in phosphate buffer and plated over phosphate/agar 8, 5 cm diameter plates to the cell densities indicated on the table. The developmental process was inspected and the time to the stage of tight aggregates was determined. The dash indicates no aggregation was observed for over 36 h.
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BMC Developmental Biology

ISSN: 1471-213X

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