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Table 2 cAMP measurements of cells submitted to nitrosoative and oxidative challenges.

From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development

Strain

pmol cAMP/107 cells

Wild-type

0.95 +/- 0.15

Wild-type + SNP

2.35 +/- 0.45

Wild-type + H2O2

1.85 +/- 0.22

acaA -

1.02 +/- 0.36

acaA- + SNP

0.70 +/- 0.02

acaA- + H2O2

0.88 +/- 0.03

yakA -

1.11 +/- 0.20

yakA- + SNP

1.15 +/- 0.15

yakA- + H2O2

1.08 +/- 0.06

keaA -

1.01 +/- 0.08

keaA- + SNP

1.32 +/- 0.27

keaA- + H2O2

1.26 +/- 0.16

  1. Exponentially growing cells were diluted to 1 × 106 cells/ml and incubated with 500 μM SNP for 24 h or H2O2 for 12 h. Cells were counted, aliquots of 5 × 106 cells were collected and the cell pellets were frozen for cAMP measurements. The values represent the mean +/- s.e.m. for 3 independent experiments.