Figure 6
From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development
keaA deficient cells development is delayed when cells are starved in agar or filters. Development was observed for wild-type and keaA deficient cells developed in phosphate-agar (A) and nitrocellulose filters (B). The images were taken at the indicated time points of starvation. (C) Wild-type cells and keaA deficient cells were developed on nitrocellulose filters and collected when cells were growing, aggregating and culminating. Samples of total RNA were analyzed on northern blots using fragments of pkaC and IG7, a constitutively expressed gene, as probes.