Figure 5
From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development
keaA deficient cells are aggregation deficient when grown in association with bacteria. Development was observed for wild-type and keaA deficient cells grown in agar in the presence of Klebsiella aerogenes. (A) A detail of the general appearance of the colonies is shown. (B) The time from clearing plate formation (beginning of starvation) to aggregation, culmination and fruiting body formation is shown from top to bottom. (C) Wild-type and keaA deficient cells clearing plates were prepared and cells were collected when they were growing, aggregating and culminating. Samples of total RNA were analyzed on northern blots using fragments of pkaC, carA and IG7, a constitutively expressed gene, as probes.