Figure 4
From: KeaA, a Dictyostelium kelch-domain protein that regulates the response to stress and development
keaA expression is induced by growth and development. Quantitative PCR was used to determine keaA expression levels during growth and development. (A) Wild-type cells were developed on phosphate buffer on Millipore filters and collected after 2 and 12 hours of development. The point 0 h represents wild-type cells in the early exponential phase of growth. The ratios are relative to transcript levels detected at 0 h (1 × 106 cells/mL). (B) Wild-type cells were grown in the presence of bacteria and collected by differential centrifugation after 44, 47 and 50 hours from plating. At 50 h plates were cleared of bacteria and development started. The ratios are relative to transcript levels detected at 44 hours. The P value was calculated in relation to the sample 44 h (reference sample). The results are representative of four different experiments.