Figure 4

MyHC immunoreactivity is lost and Z-disc formation recovers in dit. Confocal analysis of wild type (left panels) and dit embryos (right panels) stained with MyHC A4.1025, α-actinin and titin T12 antibodies. (A, C, E) A-bands and Z-discs are well-organized in wild type somites at stage 43. (B) Sarcomeric myosin staining in dit is virtually absent at these stages, but Z-disc-containing sarcomeres are observed (D, F). Phalloidin stained thin filaments are present but disorganized in dit somites.