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Figure 3 | BMC Developmental Biology

Figure 3

From: Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker

Figure 3

Myofibrillogenesis is disrupted and sarcomere formation is delayed in dit. Confocal analysis of wild type (left panels) and dit embryos (right panels) for sarcomeric myosin heavy chain (MyHC) and Z-disc (α-actinin) immunoreactivity. (A, C) Anterior somites of stage 28 wild type embryos show sarcomeric myosin (MyHC) organized into A-bands separated by regularly spaced Z-discs (α-actinin). (B) Anterior dit somites show disorganized MyHC staining, and (D) α-actinin staining is punctate, with occasional ordered Z-bodies consistent with the appearance of incipient sarcomeres (arrowheads). (E) More posteriorly at st. 28, α-actinin staining shows wild type somites 15-16 (arrow indicates intersomitic boundary) are beginning to organize Z-discs (arrowheads), while (F) dit somites at this level show only background staining (arrow indicates intersomitic boundary). Scale bars 10 μm.

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