Figure 1
From: Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker
dicky ticker maps to a lesion in the myosin co-chaperone unc45b. (A) Pools of gynogenetically derived wild type and dit embryos were assayed for linkage to polymorphisms near the 10 X. tropicalis chromosomes. Clear linkage (*) was observed at Linkage Group 6 (Chromosome 2) (B) Linkage analysis using SSLP and SNP markers with 562 mutant embryos placed the dit locus in a 230 kb interval of Scaffold_72 containing four gene models including unc45b. (C) cDNA sequence of dit unc45b revealed a thymidine to cytidine transition, substituting Arg for Cys779 in the UCS domain. (D) Cys779 is phylogenetically conserved in unc45b orthologs from Drosophila (Dm) to human (Hs).