Figure 1
From: Deletion of Irs2 causes reduced kidney size in mice: role for inhibition of GSK3β?
IRS2 is detected in mammalian kidney. Semi-quantitative (A) and quantitative TaqMan (B) PCR was performed using RNA from renal poles of 5-6 wk Irs2+/+ (empty bars), Irs2+/- (striped bars) and Irs2-/- mice (filled bars) and Irs2 specific oligonucleotides as described in Material and Methods. ΔΔCt values were calculated by subtracting the Ct values for IRS2 by those for the 18S control performed simultaneously, and fold change was then calculated by setting the wild-type liver group value to 1. Data are expressed as mean +/- SEM, n = 5 for each group. Statistical significance was determined using one way ANOVA with post hoc Tukey-Kramer multiple comparisons test, *** p < 0.001. (C) Immunoprecipitation was carried out on 1 mg protein lysates from Irs2+/+, Irs2+/- and Irs2-/- kidney. Protein lysates were separated on 7.5% SDS-PAGE and probed by Western blotting with anti-IRS2 antibody.