Figure 5

Overexpression of Prox1 results in changes in junctional proteins in vivo and in vitro. (A) RT-PCR analysis was performed on yolk sacs derived from E13.5 embryos to targets such as ZO-1, Occludin and JAM-1. (B) Similarly, in vitro analysis shows that overexpresion of Prox1 in vascular endothelial cells decreases junctional protein levels. (C) Bright field microscopy of VECs transfected with Prox1 (10x mag) (D) Vascular endothelial cells that overexpress Prox1 display an increase in permeability measured by FITC-albumin transit in a Boyden chamber assay. Figure is representative of three experiments with all samples run in triplicate. Error bars represent standard deviation of the mean. P < 0.05.