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Figure 3 | BMC Developmental Biology

Figure 3

From: Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature

Figure 3

Analysis of reprogramming in yolk sacs from Prox1 double transgenic embryos. (A) Analysis of control and DT embryos at E10.5 (lanes 1 and 2) and yolk sacs at E10.5 or E12.5 (lanes 3 to 6) for prox1 expression by RT-PCR. (B) Yolk sacs from E13.5 control and DT embryos were analyzed using RT-PCR for the expression of lymphatic associated markers such as CyclinE2, Stat6, VEGFR-2, VEGFR-3 and Neuropilin-1 (NRP1). -ve represents no template control. (C) Similarly, yolk sacs from E12.5 control and DT embryos were analyzed by western blot for the expression of VEGFR-2, VEGFR-3, Tie2 and Neuropilin-2 (NRP2).

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BMC Developmental Biology

ISSN: 1471-213X

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