Figure 5
From: Analysis of inhibitor of apoptosis protein family expression during mammary gland development
XIAP down-regulation in response to growth factor withdrawal. Primary mammary epithelial cells (MECs) were cultured in completed media (lanes 1 and 4), serum starved (lanes 2 and 5) or serum starved in the presence of 10 μM Iressa (lanes 3 and 6). (A) Whole cell lysates were immunoblotted with antibodies shown (i). XIAP protein levels relative to calnexin were calculated using Li-cor Odyssey system (ii). (B) Apoptosis was scored by counting the percentage of apoptotic nuclei. Results shown represent the average of 3 experiments +/- S.E.M. (*indicates p < 0.05; *** indicates p < 0.01).