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Figure 3 | BMC Developmental Biology

Figure 3

From: Analysis of inhibitor of apoptosis protein family expression during mammary gland development

Figure 3

Protein expression during mammary gland development. Mammary gland total protein from 3 mice (a, b, and c) was immunoblotted with the antibodies shown (P - pregnancy (days); L - lactation (days) and I - involution (hours)). (A) Phospho-stat3 is a marker of the onset of involution. Blot was stripped and re-probed with total stat-3 as a loading control (i). Full-length caspase-9 represents the inactive zymogen. Cleaved caspase-3 represents the active form (ii). Claudin-7 is a loading control for epithelial cell content between P18 days and I48 hours (iii). (B) Membranes were probed for XIAP, cIAP1 and cIAP2.

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