Figure 1
From: Analysis of inhibitor of apoptosis protein family expression during mammary gland development
IAP family members expressed during mammary gland development. Total RNA extracted from mammary gland tissue of virgin (V), lactation day 6 (L6), involution day 4 (I4) and 2 × involution day 2 (I2 & I2A) mice was subjected to reverse transcription. Control reactions were performed simultaneously in which the reverse transcriptase (RT) was omitted. cDNA was analysed by PCR with primers specific to each IAP (expected PCR product size is shown in brackets). GAPDH primers were used as a positive control (M - DNA ladder, -ve C - no cDNA, *- primer dimer products).