zGAK and zAux arose from gene duplication. (A) A phylogenetic analysis of the amino acid sequences of auxilins and GAKs without their kinase domains. The alignment was performed using Geneious software (Biomatters). (B) Alignment of intron positions in the PTEN region of auxilin and GAK orthologs. The residues shared by all are shaded in yellow and the residues conserved in mammals and vertebrates are shaded in orange. The presence of an intron is indicated by a solid line, and the phase is indicated by a number inside the triangle. ^ indicates an intron that is conserved in honeybee but not in Drosophila, whereas * indicates an intron that is conserved in fly but not in honeybee. Am: Apis mellifera, Dm: Drosophila melanogaster, Gg: Gallus gallus, Hs: Homo sapiens, Mm: Mus musculus, and Dr: Danio rerio.