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Figure 6 | BMC Developmental Biology

Figure 6

From: Tgfβ2 and 3 are coexpressed with their extracellular regulator Ltbp1in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos

Figure 6

Effect of Tgfβ treatments on mesodermal proliferation in early limb buds. (A) Representative flow cytometry histograms to evaluate proliferation in limb bud explants of stage HH20/21 cultured for 18 hours in control media or in a medium supplemented with 0.033 μg/ml Tgfβ1 after labeling with Edu. (Left): control unlabeled sample; (middle): control sample untreated with Tgfβ; (right) Tgfβ treated sample. The percentage of labeled cells is indicated in the histogram. (B) Bars graph summarizing the rate of cell proliferation in control and treated limb explants.

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BMC Developmental Biology

ISSN: 1471-213X

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