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Figure 2 | BMC Developmental Biology

Figure 2

From: Tgfβ2 and 3 are coexpressed with their extracellular regulator Ltbp1in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos

Figure 2

Expression levels of different members of transforming growth factor β subfamily in the limb bud. (A-B) Charts in A and B show the relative level of expression for the members of the Tgfβ subfamily (A) and for Activin subunits βa and βb (B). Note that tgfβ1 gene expression is the lowest of the three members of the family either at early limb bud stages (HH20; bars on the left in A) or at intermediate stages of limb development (HH24; bars on the right in A). (B) Activin βa and Activin βb displayed almost undetectable levels of expression by QPCR at the same stages of limb development. Nodal expression was totally absent and is not shown on the chart. All data was analyzed by real time QPCR and tgfβ1 expression at HH20 was used as calibrator. (*) p-value ≤ 0,05 or (**) p-value ≤ 0.01 or (***) p-value ≤ 0.001 using HH20 tgfβ1 expression level as calibrator.

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BMC Developmental Biology

ISSN: 1471-213X

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