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Figure 1 | BMC Developmental Biology

Figure 1

From: Tgfβ2 and 3 are coexpressed with their extracellular regulator Ltbp1in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos

Figure 1

pSmad 2 and 3 distribution during early limb development. (A) Immunolabeling for p-Smad2 in limb buds at stage HH24 showing negative labeling of the central chondrogenic mesenchyme (c) in contrast with the subectodermal mesoderm and the intense positivity of the AER. (B) Detailed view of the distal tip of the limb bud at stage HH24 showing positive nuclear p-smad 3 immunolabeling (green) in the AER and distal mesenchyme contrasted with cytoplasmic phalloidin-TRITC labeling (red). B' shows only the green channel in B to see the positive nuclear labeling. (C) Detailed view of pSMAD3 nuclear labeling (green) counterstained with phalloidin (red). C' shows only green channel in C.

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BMC Developmental Biology

ISSN: 1471-213X

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