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Figure 9 | BMC Developmental Biology

Figure 9

From: Drosophila mitoferrinis essential for male fertility: evidence for a role of mitochondrial iron metabolism during spermatogenesis

Figure 9

Male fertility of hypomorph P-element mutants depends on food iron levels. Female Df(3R)ED6277/TM6c flies were crossed to male flies of the genotypes dmfrnSH115/TM6c, dmfrnEY01302/TM3 and dmfrnBG00456/TM6c. Eggs were laid on normal potato fly food (N), on low iron potato fly food (140 μM BPS) or on high iron potato fly food (~2.5 mM Fe3+ as FAC) for three days. (A) Adult male offspring of the indicated genotype were mated to w1118virgin females (one male was crossed to two females) to quantify male fertility. Numbers in parentheses show the total number of single crosses per genotype. (B-G) Testes of flies reared on low iron food as observed under phase contrast (all images are of the same scale). Testes of TM6c/Df(3R)ED6277 flies (B), dmfrnBG00456/Df(3R)ED6277 flies (C and D), dmfrnEY01302/Df(3R)ED6277 flies (E and F) and dmfrnSH115/Df(3R)ED6277 flies (G) are small and contain, if any, few elongated spermatids.

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