Figure 5

Spermatid elongation defects in testis of dmfrn^SH115flies. Nuclei were stained with DAPI (blue) and actin filaments were stained with rhodamine phalloidin (red). (A) Nuclei of elongated spermatids in WT testis are needle shaped and arranged in parallel (arrow). Individualization complexes (arrow heads) are visible as parallelz arranged cone-shaped structures. (B) Nuclei of elongated spermatids in testis of dmfrn^SH115flies are needle shaped but scattered (arrows). Individualization complexes were not observed. (C) Higher magnification of the base of a testis from dmfrn^SH115flies, showing the scattered, but needle shaped nuclei of elongated spermatids. Two nuclei bundles are associated with f-actin structures (arrows), which might be newly forming individualization complexes. (D) Parallel, needle shaped nuclei (arrows) and individualization complexes (arrow heads) are observed in dmfrn^venusB32/+; dmfrn^SH115flies. Images were acquired by confocal laser scanning microscopy and represent optical slices through testes.