Figure 3

Testes of dmfrn^SH115flies show defects during spermatogenesis. Testis from a heterozygous dmfrn^SH115/TM6c fly with mature motile sperm (A arrow head). Testes from homozygous dmfrn^SH115flies lack mature sperm, can be smaller (B) than WT, often have fewer elongated spermatids (B and C) or can look similar to WT testes (D). Phase contrast of testes squashes of w¹¹¹⁸(E, G, I and M) and dmfrn^SH115flies (F, H, J, K, L, L', N and N'). Spermatocytes (F) and onion stage spermatids (H) of dmfrn^SH115tesis did not show any obvious defects and are indistinguishable from WT spermatocytes (E) and onion stage spermatids (G). Early elongating spermatids of dmfrn^SH115testis show signs of delayed elongation (J and K; the arrow indicates the dark spot on the nucleous, which is characteristic for elongating spermatids, as can be seen in WT (I)). Spermatids of dmfrn^SH115testes frequently contained white spherical objects (L and L'). Membrane blebbing was observed on mitochondrial derivatives of elongating spermatids of dmfrn^SH115testes (N and N' arrow) but not on WT testes (M, arrow)