Figure 1

The Drosophila mitoferrin ( dmfrn ) gene region and the genomic rescue construct dmfrn^venus A: P-element insertion sites and deletions in dmfrn. The gene dmfrn is encoded on the (-)strand and the coding sequence of lacZ in P-element P{lacW}mfrn^SH115 is encoded on the (-) strand as well (see Additional file 1 Figure A1A and A1D). B: The genomic rescue construct dmfrn^venus contains the gene region of dmfrn including the intergenic region between dmfrn and Gp93. The stop codon of dmfrn was substituted by the coding sequence of the fluorescent marker venus, resulting in the expression of a C-terminally venus-tagged dmfrn protein. The 5' and 3' inverted repeats of the P-element used for generation of transgene flies by P-element transposition are indicated by the black boxes.