Gene trap mutation of murine Outer dense fiber protein-2 gene can result in sperm tail abnormalities in mice with high percentage chimaerism

Table 1 XL169- and RO072- derived chimaeric male Breeding Results

male XL169 mice analyzed^a	total # offspring produced by males analyzed	agouti offspring produced^b	heterozygotes produced
low-medium percentage chimaeric males^c	494	162	6
high percentage chimaeric males^d	0	N/A^e	N/A
male RO072 mice analyzed	total # offspring produced by males analyzed	agouti offspring produced	heterozygotes produced
low-medium percentage chimaeric males^f	301	46	8
high percentage chimaeric males^g	75	11	2

Notes:
a) chimaerism was determined by coat color (agouti): over 90% chimaeric is classified as "high", below 90% as "low-medium".
b) agouti offspring can have the Odf2^+/+ genotype (phenotypically wt mice) or the Odf2^+/- genotype (heterozygote). In agouti offspring one Odf2 allele derives from the C57Bl/6 background and the other allele (either the normal Odf2 allele, or the genetrap-interrupted Odf2 allele) derives from the XL169 ES background
c) 13 males of low-medium % chimaerism were analyzed by breeding
d) 5 males of high % chimaerism were analyzed by breeding
e) N/A = not applicable.
f) 7 males of low-medium % chimaerism were analyzed by breeding
g) 2 males of high % chimaerism were analyzed by breeding

ISSN: 1471-213X