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Figure 2 | BMC Developmental Biology

Figure 2

From: Gene trap mutation of murine Outer dense fiber protein-2 gene can result in sperm tail abnormalities in mice with high percentage chimaerism

Figure 2

Odf2-β-geo RNA is expressed in XL169 chimaeric mice. A) We analyzed Odf2-β-geo RNA expression in RNA isolated from primary fibroblasts XL169 chimaeric ear clip cultures using RT PCR. Primers were specific for Odf2-β-geo (lanes 2 - 5; "target") or for wild type Odf2 (lanes 6 - 9; "normal"). Lane 1, DNA markers; lanes 2 , 3, 6 and 7, chimaeric males; lanes 4, 5, 8 and 9, Odf2+/- F1 offspring . B) Northern blotting analysis was used to compare expression levels of wild type Odf2 RNA and Odf2-β-geo RNA in testis from XL169 and RO072 chimaeric mice. Top panel: blots were analyzed using a geo-specific probe that only detects Odf2-β-geo RNA ("RO072" and "XL169"). Bottom panel: RNA blots were analyzed using an Odf2-specific probe, which detects both Odf2 RNA ("wt") and Odf2-β-geo fusion RNA ("RO072" and "XL169"). The source of RNA was: lane 1, XL169 chimaeric male; lane 2, C57Bl/6 male; lane 3, RO072 chimaeric male.

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