Figure 1

Genomic analysis XL169 chimaeric mice and offspring. A) DNA was extracted from tail snips of XL169 chimaeric mice and C57BL/6 control mice and analyzed by PCR for the insertion of the β-geo cassette between Odf2 exons 4 and 5. Each DNA sample was analyzed by primers specific for actin (lanes 1, 2, 3, 4, 5 and 6: "a") and primers specific for Odf2-β-geo (lanes 1', 2', 3', 4', 5' and 6'; "o"). Lane 7 shows DNA markers. The source of DNA was as follows: lanes 1 and 1', C57BL/6 (which does not have Odf2-β-geo DNA); lanes 3 - 6', XL169-derived chimaeric male mice with various percentage chimaerism. B) PCR was used to detect Odf2-β-geo DNA in testicular DNA of F1 offspring resulting from matings of XL169 chimaeric males with normal females. Each DNA sample was analyzed by primers specific for actin (lanes 2, 3 and 4; "a") and primers specific for Odf2-β-geo (lanes 2', 3' and 4'; "o"). Lane 1 shows DNA markers. The source of DNA was: lanes 2 and 3, agouti F1, which did not inherit Odf2-β-geo DNA; lanes 4 and 5, chimaeric male; lanes 6 and 7, agouti Odf2^+/- offspring.