Figure 6

Effects of Dicer1 ablation on apoptosis in the testis. (A) Immunofluorescence for cleaved Caspase3 (CASP3) was performed on sections of testes at 18.5 dpc, P0, P2, and P5. Green nuclear staining represented positive signals for apoptotic cells and blue staining was the DAPI nuclear counterstain. Double staining of CASP3 (green) and TRA98 or laminin (magenta) was performed on testes from P0 (B and D) and P2 (C and E) KO testes. Dotted lines mark the testis cords. Arrowhead = apoptotic cells outside the testis cord. Arrow = apoptotic germ cells. Scale bars represent 100 μm.