Figure 5
Effects of Dicer1 ablation on differentiation of germ cells, Sertoli cells, and Leydig cells in the testis. Testes from control (WT) or Sf1/Cre;Dicer1loxP/loxP (KO) newborns (P0), Day 2 (P2), and Day 5 (P5) neonates were collected for (A) immunofluorescence for germ cell nuclear marker TRA98 (magenta), Sertoli cell marker SOX9 (green) and DAPI counterstain (blue) and (B) immunofluorescence for Leydig cell marker HSD3b (magenta), proliferation marker Ki67 (green), and DAPI (blue). Higher magnification (2× of the original figure) of the proliferating cells for P0 is shown in the inlets. Scale bars represent 100 μm.