Figure 5

Fate of embryonic blood cells. (A-T) Blood smears (A-D, I-L and Q-T) and lymph glands (E-H and M-P) of third instar larvae. The Act > FRT > CD2 > FRT > GAL4 cassette and the UAS-FLP and UAS-GFP transgenes were used to permanently label the cells that express sn-Gal4 or gcm-Gal4. Immunostaining against GFP (green, also displayed in white on right panel of each blood smear) was used to monitor gcm-Gal4, UAS-GFP or sn-Gal4, UAS-GFP expression. In situ hybridization against α-ps4 (red) was used to reveal lamellocyte differentiation. Nuclei were counterstained with DAPI. GFP labeling alone is shown to the right. (A-H) larvae raised in wild-type conditions, (I-P) larvae carrying a UAS-dsRNA transgene against ush, (Q-T) larvae submitted to parasitization by L. boulardi. (A, E, I, M, Q) gcm-Gal4, UAS-GFP; Act > FRT > CD2 > FRT > GAL4; (B, F, J, N R) UAS-FLP; gcm-Gal4, UAS-GFP; Act > FRT > CD2 > FRT > GAL4; (C, G, K, O, S) sn-Gal4, UAS-GFP; Act > FRT > CD2 > FRT > GAL4; (D, H, L, P, T) UAS-FLP; sn-Gal4, UAS-GFP; Act > FRT > CD2 > FRT > GAL4;