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Table 4 Development of reconstructed embryo in relation to the cell injection location.

From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish

 

Embryo development (%)

 

Mid-blastula

24 h

Hatching stage

Normal morphology at hatching

Cell location

Mean

Min - max

Mean

Min - max

Mean

Min - max

Mean

Min - max

Shallow

59.3 ± 5.9 (a)

50 - 70

16.5 ± 8.6 (a)

5 - 30

14.5 ± 7.0 (a)

7 - 23

2.0 ± 2.1 (a)

0 - 5.0

Deep

22.9 ± 4.7 (b)

15 - 30

0.0 ± 0 (b)

-

0.0 ± 0 (b)

-

0.0 ± 0 (b)

-

  1. Developments after oocyte activation are expressed as a percentage of the spawn quality control in the same spawn at the same stage (mean ± SE, n = 7 spawns); 40 oocytes were tested in each nuclear transferred sample. Injected oocytes were incubated for 30 min prior to activation.