Figure 4
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
Development of reconstructed embryo in relation to the incubation time prior to oocyte activation. Developments are expressed as a percentage of the spawn quality controls in the same spawn at the same stage (mean ± SE, n = 4 to 6 spawns). Forty oocytes were tested in each spawn. Cells in 10 pL TCF were introduced in the shallow position and oocytes were activated after 0 min (white bars), 30 min (dotted bar), and 60 min (black bar). (a) (b): Different letters within one stage indicate significant differences (p < 0.05).