Figure 2
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
Aggregation of fertilized eggs incubated in SOF with STI prior to activation. Oocytes were incubated for 30 min in soybean trypsin inhibitor (STI) 0.1 mg/mL in synthetic ovarian fluid (SOF) prior to fertilization. The picture was taken 5 min (A) and 24 h (B) after fertilization. Egg aggregation impaired embryo development as seen at 24 h by the darkening of dead embryos, although live embryos (translucent) are still visible. Scale bar = 500 μm.