Skip to main content
Figure 4 | BMC Developmental Biology

Figure 4

From: The homolog of Ciboulot in the termite (Hodotermopsis sjostedti): a multimeric β-thymosin involved in soldier-specific morphogenesis

Figure 4

Western blot and in situ hybridization to detect HsjCib expression. A: Western blot analysis detected by anti-Drosophila Cib antibody against head homogenates of the termite. The antibody detected strong bands in the 14 d (14 days after JHA application) lane and weak bands in the PE (normal pseudergate) lane, which were presumed to correspond to isoforms of HsjCib. B-E: In situ hybridization of the HsjCib mRNA in newly formed mandible (14 d stage). Transverse paraffin sections (6 μm) were subjected to in situ hybridization with antisense (B and D) and sense (C and E) DIG-labeled RNA probes. Bars indicate 200 μm (B and C) and 20 μm (D and E), respectively. The expression of HsjCib was observed in the mandibular epidermis.

Back to article page