Figure 4From: The homolog of Ciboulot in the termite (Hodotermopsis sjostedti): a multimeric β-thymosin involved in soldier-specific morphogenesisWestern blot and in situ hybridization to detect HsjCib expression. A: Western blot analysis detected by anti-Drosophila Cib antibody against head homogenates of the termite. The antibody detected strong bands in the 14 d (14 days after JHA application) lane and weak bands in the PE (normal pseudergate) lane, which were presumed to correspond to isoforms of HsjCib. B-E: In situ hybridization of the HsjCib mRNA in newly formed mandible (14 d stage). Transverse paraffin sections (6 μm) were subjected to in situ hybridization with antisense (B and D) and sense (C and E) DIG-labeled RNA probes. Bars indicate 200 μm (B and C) and 20 μm (D and E), respectively. The expression of HsjCib was observed in the mandibular epidermis.Back to article page