Figure 3

Proliferation of human ES cells on an hE-cad-Fc-coated surface. (A) The proliferative activity of ES cells was evaluated in surfaces coated with either Matrigel or hE-cad-Fc-coated. H9 cells were seeded on Matrigel- or hE-cad-Fc-coated dishes and cultured in MEF conditioned media (CM) or mTeSR1, and the cell number was counted after staining with WST-1 reagent. The data indicate means ± SEM of individual experiments (n = 5). (B) Growth characteristics of human ES cells cultured in various conditions. The data represent means ± SEM of five individual experiments. (C) The number of BrdU-, phospho-histone H3-, and activated caspase 3-positive cells were determined by immunohistochemistry after 2 days culture on a Matrigel- or hE-cad-Fc-coated surface. The data represent means ± SEM of five individual experiments. N.S. = not statistically significant.