Gene ontology (GO) analyses of the genes that were differentially expressed between rSey2/ rSey2 and WT rat hindbrains at E11.5. Annotated genes were selected by t-test using the cross-gene error model (p < 0.05). The genes are categorized by the following GO terms: cellular component (144, A), molecular function (160, B) and biological process (160, C), and are displayed in doughnut charts. Internal color arcs indicate the total rates for each category: (A) membrane (green, 52.1%), cytoplasm (blue, 29.9%) and nucleus (red, 16.0%); (B) signal transducer (green, 25.6%), catalytic (blue, 25.6%), binding (red, 25.0%) and transporter (yellow, 16.9%) activities; and (C) development (red, 26.9%), signal transduction (green, 20.0%), transport (yellow, 18.1%), and metabolism (blue, 16.9%). The details of the annotated genes and GO terms are shown in Additional file 3.