Figure 7
cpnA - cells can differentiate into morphologically normal stalk and spore cells. cpnA- cells expressing GFP (5%) and wild-type cells (95%) were mixed, plated on black filters in starvation buffer at 5 × 107 cells/mL, and allowed to develop for 24 hours. Fruiting bodies were removed from the filter and placed in glass bottom dishes. Spores (A, C) and stalks (B, D) were imaged with DIC (A, B) and fluorescence (C, D) microscopy using a Nikon fluorescence microscope at 600× magnification (scale bar = 40 μm).