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Figure 5 | BMC Developmental Biology

Figure 5

From: Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development

Figure 5

Wild-type cells differentiate into stalk or spore cells when mixed with cpnA - cells. Wild-type cells expressing GFP (10%) were mixed with cpnA- cells (90%), plated on black filters in starvation buffer at 5 × 107 cells/mL, and allowed to develop for 24 hours. Fluorescent images of chimeric slugs (A) and fruiting bodies (B) were taken with a Leica dissecting microscope at 50× magnification (scale bar = 0.5 mm). (C) Wild-type cells expressing GFP and cpnA- cells were mixed at various ratios and allowed to develop. Fruiting bodies were removed from the filter, placed on glass bottom dishes, and imaged with a Nikon fluorescence microscope. The percentage of fluorescent spores from each ratio was calculated five times and averaged. Error bars indicate standard error.

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