Figure 6

Hypodermal cell defects in ten-1(ok641) embryos. All animals shown are ten-1(ok641) mutants carrying the ajm-1::gfp transgene, which allows the visualization of the adherence junctions that surround the hypodermal cells. (A) A 1.5-fold embryo that is developing normally. Note the seam cells indicated by asterisks, and the ongoing hypodermal cell fusions on the dorsal side (discontinuous lines of GFP expression). (B) An example of a 2-fold embryo in which the posterior half has developed abnormally. Note the poorly organized pattern of ajm-1::gfp expression. (C) An early 3-fold stage embryo that is developing normally. Note the evenly spaced seam cells (asterisks) and the symmetrical distribution of the ajm-1::gfp pattern. (D) An early 3-fold embryo in which the posterior half has developed abnormally. Note in particular the meandering and disorganized pattern of ajm-1::gfp distribution. (E) Ventral aspect of an L1 larva that has developed normally. Again, note the regular shapes of the hypodermal cells. (F) Ventral aspect of an L1 larva with a deformity in its posterior half, which corresponds to a misshaped hypodermal cell (arrow). (G) DIC image of a ten-1(ok641) L1 larva with focus on the two muscle quadrants with normal appearance (asterisks) in spite of the posterior deformity (arrow). (H) DIC image of a ten-1(ok641) L1 larva with focus on the deformed intestine (asterisks) and posterior deformity (arrow).