Figure 2

Expression of ten-1a/b transcriptional reporters. (A-E) Embryonic expression of ten-1a::gfp. The asterisk in (A) indicates the anterior cluster of GFP-positive cells in a ~150 min embryo. Arrowheads in (B) indicate intercalating hypodermal cells, while the asterisk indicates a cluster of pharyngeal precursor cells in a ~300 min embryo. (C-E) Shows that pharyngeal cells (p), intestinal (i) and some neurons (n) express ten-1a::gfp in ~350 min (dorsal view), 1.5-fold, and 2-fold stage embryos, respectively. (F-G) Show a L1 larva and the head of an adult, respectively. Note expression in the marginal cells (mc1 and mc3), head ganglion neurons (hg), the intestine (i) and the M2 pharyngeal neuron (M2). (H-J) Show a L1 ten-1(ok641) mutant larva expressing ten-1a::gfp. Note the deformed posterior end (indicated by arrowhead in H), and the poor connection between the posterior intestinal cells (indicated by arrowheads in J). (I) is an overlay of (H) and (J). (K-O) Embryonic expression of ten-1b::gfp. The asterisk in (K) indicates the anterior cluster of GFP-positive cells in a ~150 min embryo. Arrowheads in (L-M) indicate anterior and posterior clusters of hypodermal cells in ~300 min embryos shown from a dorsal or lateral perspective, respectively. (N-O) ten-1b::gfp expression in several neurons of the head and tail in 1.5 and 2-fold embryos, respectively. (P-T) Show expression of ten-1b::gfp ten-1(ok641) mutant embryos. Note abnormal development of the embryos and the persistence of strong GFP expression in dorsal hypodermal cells (asterisks in R; arrowheads in S and T) at stages where the expression is declining or lost from wild-type embryos. (U) Shows a L1 larva, with several head ganglion neurons (hg) and tail neurons (n) expressing GFP. (V) Shows an adult pharynx expressing ten-1b::gfp and stained with DiI, which labels the taste sensory amphid neurons (red). Note the absence of overlap between the DiI and GFP signals. (W-X) Show the head of an adult ten-1(ok641) mutant expressing ten-1a::gfp. Note the presence of GFP-positive axons forming an ectopic nerve ring anterior to the metacorpus (arrowheads), which is never seen in wild-type controls. The scale bar in (A) represents 10 μm and applies to all panels except (H-J), which have a separate scale bar.