Figure 1

Mapping of ten-1(et5). (A) Shows part of the map for chromosome III, including the positions of the genetic markers used to map mnm-5/ten-1(et5). Also shown are the structures of 16 informative recombinants among the F2 progeny of dpy-17 ten-1(et5) unc-32 mutants crossed with the Hawaiian strain CB4860. Open circles refer to the N2 allele at each locus. (B) and (C) Show an enlarged region of chromosome III and the locations of five fosmids that were tested for their ability to rescue the ten-1(et5) mutant. The full name for each fosmid includes the prefix "WRM06", and the rescuing fosmid WRM064dE07 is highlighted in red. (D) Partial restriction map of the WRM064dE07 fosmid, which contains the genes ten-1, srv-1, zak-1 and R13F6.5. The restriction enzyme sites AhdI, EcoNI, PstI and XhoI sites are respectively indicated as A, E, P and XhoI. (E) Structure of the ten-1 gene showing the two transcription starts, the regions deleted in the tm651 and ok641 alleles and the position of the et5 c>t nucleotide substitution. (F) Domain structure of the TEN-1 protein.