Figure 1

Ablim1 is a novel asymmetrically expressed locus. (a) Diagram representing the Ablim1 transcript. The relative positions of the probes used for WISH analysis are marked at the top of the panel. The exon structure is indicated in the middle and the two major protein isoforms of Ablim1 are indicated at the bottom; the long isoform has 4 lim domains that the short form lacks. (b-m) Comparison of embryonic expression patterns of Ablim1 using probes to different mRNA regions. (b-e) Developmental expression of Ablim1 using the full length cDNA probe. (b) patchy yolk sac expression at 6.5 dpc, indicated by arrowhead, (c) a ring of expression in the yolk sac (arrowhead), head and node (open arrowhead) at 7.5 dpc, (d), symmetric expression in the head and yolk sac in addition to asymmetric expression is the lateral plate seen at 8.5 dpc, (e) expression in the heart, head and somites at 9.5 dpc. Expression at 8.5 dpc was assessed with Ex2-6 (f, j), Ex8-25 (g, k), 3'UTRa (h, l) and 3'UTRb (I, m). (f-i) flat mounted 8.5 dpc embryos dissected from their yolk sacs, visualised ventrally. (j-m) 8.5 dpc embryos within yolk sacs visualised from the posterior. The posterior most extent of LPM expression is indicated by a closed arrowhead and node expression is indicated by an open arrowhead. Panels (b), (c) and (e) are lateral views. In other panels Left (L) and Right (R) are as marked and are the same for each row of panels.