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Figure 3 | BMC Developmental Biology

Figure 3

From: Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters

Figure 3

Endogenous versus exogenous promoters in transactivator and responder constructs. Only when Tribolium core promoters are used in the responder and transactivator constructs, activation of the reporter gene is observed via in-situ hybridization. Exogenous promoters are not capable of driving reporter gene expression. (a-h) Transactivator line Tc-hsp-GAL4Δ#1 was crossed to different responder lines and in-situ hybridization was performed with the respective antisense RNA probe. (a, b) In the positive controls (UAS-Tc-bhsp-tGFP) ubiquitous expression of tGFP is detected. In contrast, no reporter activity is detected in constructs utilizing a Drosophila core promoter (UAS-Dm-hsp-LacZ) (c, d) or an artificial "super core promoter" (UAS-SCP-DsRed) optimized for vertebrate cells (e, f). (g, h) A construct containing both the Drosophila basal heat shock promoter and the Tribolium hairy promoter (UAS-Dm-hsp-Tc-bh-EYFP) shows activation predominantly in the nervous system of advanced embryos. (i-k) The Drosophila heat shock promoter driving Gal4 shows no activity in one insertion line (i) while in the other some isolated cells show expression of the reporter (k).

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